Generator

Part:BBa_K3962342:Design

Designed by: Siheng Li   Group: iGEM21_Leiden   (2021-10-11)


arabinose-mediated inducible expression of toxin CcdB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 1902
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1352
    Illegal SapI site found at 961


Design Notes

pBAD has been intensively investigated by previous iGEM projects and has tight regulation upon downstream genes. It is the most suitable promoter for regulating toxin genes and preventing their toxic effects because glucose can be used to repress its activity. Codon optimization was performed for the expression of the gene in E. coli.



Source

All parts come from the iGEM registry.


References